746912 Laboratory Course on real time Polymerase Chain Reaction (PCR)

summer semester 2014 | Last update: 10.01.2014 Place course on memo list
746912
Laboratory Course on real time Polymerase Chain Reaction (PCR)
VU 1
2
Block
every 3rd semester
English

Students will learn how to perform qPCR.

This course aims to introduce students to real-time or quantitative PCR (qPCR). A theoretical session discusses the science underlying qPCR and provides an overview over the different commercially available chemistries. Advantages and disadvantages of each system are pointed out. In addition, critical aspects of primer design will be discussed. The practical session introduces the students to the most common applications of qPCR, namely relative and absolute quantification. Further, students follow a protocol designed to cover all steps starting from preparation and quality control of samples (by Microfluidics-based analysis of RNA preparation) up to the final analysis of quantitative gene expression.

Continuous assessment (based on regular written and/or oral contribution by participants).

Course examination according to § 7, statute section on "study-law regulations"

Handouts will be provided.

For the preliminary discussion an e-mail will be send to the students.
Meeting point is at the Institute for Biomedical Aging Research, Rennweg 10 in the seminar room.

Ther course will be held at the Institute for Biomedical Aging Research.

12.03.2014
12.03.2014, 9:00 - 13:00 Uhr, IBA, Seminarraum
13.03.2014, 9:00 - 12:00 Uhr, IBA, Seminarraum
14.03.2014, 9:00 - 13:00 Uhr, IBA, Seminarraum