146315 VU Biomedical Engineering 5 - Advanced: Molecular Biological Methods for Diagnosis and Therapy
winter semester 2018/2019 | Last update: 30.11.2023 | Place course on memo listBasic knowledge of cell biology, biochemistry, and molecular biology
Detailed knowledge of the most relevant analytical laboratory methods (basic molecular biology techniques and high-throughput methods)
Basic knowledge of data analysis methods and quantitative assessment of diagnostic markers and practices
· Principles of cell biology and biochemistry
o Cell, organelles, cell cycle
o Biochemicals and most important metabolic pathways
· Principles of molecular biology
o The human genome and ist variability
o Replication
o Transcription
o Translation
o Post-translational modifications
· Basic techniques in molecular biology
o Properties and isolation of nucleic acids
o Electrophoresis, blotting, hybridization
o Amplification, Cloning, PCR, RT-PCR
o Classical sequencing (ddNTP, Sanger)
o Quantitation (dot blot, qPCR, qRT-PCR)
· High-throughput technologies
o Mutational analysis (DASH, molecular beacons, chip-based methods)
o Next-generation sequencing (454, Illumina, Solid, Ion torrent)
o Transcriptomics (Chip-based, transcript-sequencing)
o Proteomics (HPLC, CE, mass spectrometry)
o Metabolomics (NMR, HPLC, GC, CE, mass spectrometry)
o Biological databases (GenBank, dbSNP, OMIM, HapMap, PIR, HMDB, ...)
· Data analysis and assessment of diagnostic methods
o S/N ratio, LOD, LLOQ, linear range, ULOQ
o Distributions, reference ranges
o Sensitivity, Sepecificity, PPV, NPV
o ROC analysis
o Uni- and multivariate statistics and data visualization (Tests, overfitting, correction, PCA, PLS-DA, volcano-plot, ...)
Continuous assessment (based on regular written and/or oral contribution by participants).
Oral examination and continuous assessment of contributions during lectures
· Friedrich Lottspeich & Joachim Engels, Bioanalytik, Springer Spektrum Akademischer Verlag, 3. Auflage 2012, ISBN 978-3827429421
· Lothar Thomas, Labor und Diagnose, TH-Books, 8. Auflage 2012, ISBN 978-3980521581
Group 0
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Date | Time | Location | ||
Wed 2018-10-24
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13.15 - 16.45 | UMIT UMIT | SR109 | |
Wed 2018-10-31
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13.15 - 16.45 | UMIT UMIT | SR109 | |
Wed 2018-11-07
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13.15 - 16.45 | UMIT UMIT | SR109 | |
Wed 2018-11-21
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13.15 - 16.45 | UMIT UMIT | SR109 | |
Thu 2018-11-29
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13.15 - 16.45 | UMIT UMIT | SR109 | |
Thu 2018-12-13
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13.15 - 16.45 | UMIT UMIT | SR109 | |
Thu 2018-12-20
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13.15 - 16.45 | UMIT UMIT | SR109 Prüfung |