LFU:online

Students will have hands-on in field (Research Station Gossenköllesee) and laboratory work related to the lecture. The students will acquire detailed knowledge on different methods (HPLC, underwater radiometry, organic carbon analyzer, spectrophotometry) used to characterize the dissolved organic matter, underwater optical properties,  effects of solar radiation and photoprotective compounds by doing measurements and experiments.

The tutorial includes field and laboratory measurements/experiments on:

1) Sources of artificial UV radiation and materials used in experiments to test the effects of UV radiation
2) Use of weighting factors from an action spectrum, normalizing an action spectrum, dose rate and dose calculations
3) Use of different sensors to measure surface and underwater UV-A and UV-B radiation, characterization of the chromophoric or colored dissolved organic matter (CDOM) by determination of the concentration of dissolved organic carbon (DOC), and the spectral absorption of the dissolved fraction. (Definitions: immersion effect, cosine response, spectral responsivity, full width half maximum).
4) Determination of mycosporine-like amino acids in planktonic organisms by HPLC
5) Effects of solar UV radiation on aquatic organisms

Spectrophometry

Spectrofluorometry

Radiometry

HPLC

Water organic chemistry (DOC)

Examination is based on an individual  report describing the activities during the field and laboratory work.

Björn, L.O. and Murphy, T.M., 1985. Computer calculation of solar ultraviolet radiation at ground level. Physiol. Vég. 23, pp. 555–561.

Dunlap, W.C., D.M. Williams, B. Chalker and A. Banaszak. 1989. Biochemical photoadaptation in vision: UV-absorbing pigments in fish eye tissues. Comp. Biochem. Physiol. 93B:601-607.

Dunlap, W. C. & Shick, J. M. (1998) Ultraviolet radiation-absorbing mycosporine-like amino acids in coral reef organisms: a biochemical and environmental perspective. J. Phycol. 34: 418-430.

Fischer JM, Fields PA, Pryzbylkowski PG, Nicolai JL, and Neale PJ. 2006 Sublethal exposure to UV radiation affects respiration rates of the freshwater cladoceran Daphnia catawba. Photochem Photobiol. 82(2): 547-550.

Hoge FE, Vodacek A, Blough NV (1993) Inherent optical properties of the ocean: retrieval of the absorption coefficient of chromophoric dissolved organic matter from fluorescent measurements. Limnol. Oceanogr. 38: 1394-1402.

Leech, D.M., and Williamson, C.E. 2001. In situ exposure to ultraviolet radiation alters the depth distribution of Daphnia. Limnol. Oceanogr. 46: 416-420.

Setlow, R. B. 1974. The wavelengths in sunlight effective in producing skin cancer: a theoretical analysis. Proc. Natl. Acad. Sci. USA, 71:3363-3366.

Shick, J. M., Dunlap, W.C., Chalker, B.E., Banaszak, A.T. & Rosenzweig, T.K. (1992) Survey of ultraviolet radiation-absorbing mycosporine-like amino acids in organs of coral reef holothuroids. Mar. Ecol. Prog. Ser. 90: 139-148.

Sommaruga, R., F. Garcia-Pichel (1999). UV-absorbing mycosporine-like compounds in planktonic and benthic organisms from a high-mountain lake. Arch. Hydrobiol. 144: 255-269.

Semester introduction for all students in the Master's Programme Ecology+Biodiversity:  Mo 4.3.2019, 11:00 - 12:00, Hörsaal B, Technikerstr. 25.