104701 VO Special Molecular Biology

winter semester 2021/2022 | Last update: 21.03.2022 Place course on memo list
VO Special Molecular Biology
VO 1

Main aim of this couse is to introduce students into the basics of biomolecular methods:

  • search in scientific data bases
  • bioinformatic analyses of DNA/mRNA
  • extraction & purification of DNA/RNA
  • synthesis of cDNA
  • polymerase chain reaction (PCR)
  • cloning of PCR fragments
  • transformation of E. coli
  • verification of clones ('Blue-white-screening', colony PCR, restriction analyses, agarose gel electrophoresis)
  • cloning of DNA construct for expression of fusion proteins

Additionally, basics of bioinformatics will be taught:

  • use of databases (ncbi, ensembl)
  • alignments/search of sequences (lalign, blast)
  • in silico cloning (pDraw or SerialCloner)
  • design of primers

Biomolecular methods, such as isolation of DNA and RNA, PCR and molecular cloning.

  • weekly schedule:
    • 1st week: cloning of two zebrafish genes (gDNA/cDNA), all together
    • 2nd week: individual cloning projects in small groups
  • RNA/DNA extraction and purification
  • cDNA synthesis via reverse transcriptation
  • in silico PCR/cloning
  • PCR based amplification of cDNA/gDNA 
  • TA cloning
  • verification of cloning via 'blue-white-screening', colony PCR or restriction analysis
  • exam after lecture
  • continuous assessment (based on regular written and/or oral contribution by participants 
  • final protocol of the practical part
  • Alberts et al., „Molecular Cell Biology“ (4th edition), ISBN-13:978-3527328246
  • Lottspeich & Engels, „Bioanalytik“ (3rd edition), ISBN-13:978-3827429421
  • T.A. Brown, „Genomes 3“ (3rd edition), ISBN-13: 978-0815341383

Basic Laboratory Course and Introduction to Molecular Biology

Preliminary meeting on October 5, 2021, 5.15 pm, lecture hall G.

Please sign in only for group 0 (=lecture).

A (preliminary) division of the courses will be done according to the preferences of participants during the preliminary meeting and according to § 6 of the curriculum:

§ 6 Allocation of places in courses with a limited number of participants

In courses with a limited number of participants, course places are allocated as follows:

  1.  Presence at the preliminary meeting (personal or representative)
  2. Date of fulfilment of the necessary admission requirement(s); students who fulfilled the ad- mission requirement(s) at an earlier time are to be given priority.
  3. Number of semesters the student has been enrolled for the Bachelor’s Programme Biology; Students who have been enrolled for a longer time are to be given priority.
  4. By lot.
to be announced
Group 0
Date Time Location
Tue 2021-10-05
17.15 - 19.00 HS G (Technik) HS G (Technik) Barrier-free
Wed 2021-10-06
17.15 - 19.00 HS E (Technik) HS E (Technik) Barrier-free
Thu 2021-10-07
17.15 - 19.00 HS E (Technik) HS E (Technik) Barrier-free
Fri 2021-10-08
17.15 - 19.00 HS E (Technik) HS E (Technik) Barrier-free