104701 VO Special Molecular Biology
winter semester 2021/2022 | Last update: 21.03.2022 | Place course on memo list104701
VO Special Molecular Biology
VO 1
1,5
Block
annually
German
Main aim of this couse is to introduce students into the basics of biomolecular methods:
- search in scientific data bases
- bioinformatic analyses of DNA/mRNA
- extraction & purification of DNA/RNA
- synthesis of cDNA
- polymerase chain reaction (PCR)
- cloning of PCR fragments
- transformation of E. coli
- verification of clones ('Blue-white-screening', colony PCR, restriction analyses, agarose gel electrophoresis)
- cloning of DNA construct for expression of fusion proteins
Additionally, basics of bioinformatics will be taught:
- use of databases (ncbi, ensembl)
- alignments/search of sequences (lalign, blast)
- in silico cloning (pDraw or SerialCloner)
- design of primers
Biomolecular methods, such as isolation of DNA and RNA, PCR and molecular cloning.
- weekly schedule:
- 1st week: cloning of two zebrafish genes (gDNA/cDNA), all together
- 2nd week: individual cloning projects in small groups
- RNA/DNA extraction and purification
- cDNA synthesis via reverse transcriptation
- in silico PCR/cloning
- PCR based amplification of cDNA/gDNA
- TA cloning
- verification of cloning via 'blue-white-screening', colony PCR or restriction analysis
- exam after lecture
- continuous assessment (based on regular written and/or oral contribution by participants
- final protocol of the practical part
- Alberts et al., „Molecular Cell Biology“ (4th edition), ISBN-13:978-3527328246
- Lottspeich & Engels, „Bioanalytik“ (3rd edition), ISBN-13:978-3827429421
- T.A. Brown, „Genomes 3“ (3rd edition), ISBN-13: 978-0815341383
Basic Laboratory Course and Introduction to Molecular Biology
Preliminary meeting on October 5, 2021, 5.15 pm, lecture hall G.
Please sign in only for group 0 (=lecture).
A (preliminary) division of the courses will be done according to the preferences of participants during the preliminary meeting and according to § 6 of the curriculum:
§ 6 Allocation of places in courses with a limited number of participants
In courses with a limited number of participants, course places are allocated as follows:
- Presence at the preliminary meeting (personal or representative)
- Date of fulfilment of the necessary admission requirement(s); students who fulfilled the ad- mission requirement(s) at an earlier time are to be given priority.
- Number of semesters the student has been enrolled for the Bachelor’s Programme Biology; Students who have been enrolled for a longer time are to be given priority.
- By lot.
to be announced
Group 0
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Date | Time | Location | ||
Tue 2021-10-05
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17.15 - 19.00 | HS G (Technik) HS G (Technik) | Barrier-free | |
Wed 2021-10-06
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17.15 - 19.00 | HS E (Technik) HS E (Technik) | Barrier-free | |
Thu 2021-10-07
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17.15 - 19.00 | HS E (Technik) HS E (Technik) | Barrier-free | |
Fri 2021-10-08
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17.15 - 19.00 | HS E (Technik) HS E (Technik) | Barrier-free |