LFU:online

Main aim of this couse is to introduce students into the basics of biomolecular methods:

• search in scientific data bases
• bioinformatic analyses of DNA/mRNA
• extraction & purification of DNA/RNA
• synthesis of cDNA
• polymerase chain reaction (PCR)
• cloning of PCR fragments
• transformation of E. coli
• verification of clones ('Blue-white-screening', colony PCR, restriction analyses, agarose gel electrophoresis)
• cloning of DNA construct for expression of fusion proteins

Additionally, basics of bioinformatics will be taught:

• use of databases (ncbi, ensembl)
• alignments/search of sequences (lalign, blast)
• in silico cloning (pDraw or SerialCloner)
• design of primers

Biomolecular methods, such as isolation of DNA and RNA, PCR and molecular cloning.

• weekly schedule:
• 1st week: cloning of two zebrafish genes (gDNA/cDNA), all together
• 2nd week: individual cloning projects in small groups

• RNA/DNA extraction and purification
• cDNA synthesis via reverse transcriptation
• in silico PCR/cloning
• PCR based amplification of cDNA/gDNA 
• TA cloning
• verification of cloning via 'blue-white-screening', colony PCR or restriction analysis

• exam after lecture
• continuous assessment (based on regular written and/or oral contribution by participants 
• final protocol of the practical part

• Alberts et al., „Molecular Cell Biology“ (4th edition), ISBN-13:978-3527328246
• Lottspeich & Engels, „Bioanalytik“ (3rd edition), ISBN-13:978-3827429421
• T.A. Brown, „Genomes 3“ (3rd edition), ISBN-13: 978-0815341383
• manual of the practical course (will be uploaded on OLAT)

Basic Laboratory Course and Introduction to Molecular Biology

Please sign in only for group 0 (=lecture).

A (preliminary) division of the courses will be done according to the preferences of participants during the preliminary meeting and according to § 6 of the curriculum:

§ 6 Allocation of places in courses with a limited number of participants

In courses with a limited number of participants, course places are allocated as follows:

1.  Presence at the preliminary meeting (personal or representative)
2. Date of fulfilment of the necessary admission requirement(s); students who fulfilled the ad- mission requirement(s) at an earlier time are to be given priority.
3. Number of semesters the student has been enrolled for the Bachelor’s Programme Biology; Students who have been enrolled for a longer time are to be given priority.
4. By lot.